Journal: Scientific Reports
Article Title: Extracellular vesicles from Paracoccidioides pathogenic species transport polysaccharide and expose ligands for DC-SIGN receptors
doi: 10.1038/srep14213
Figure Lengend Snippet: Data were normalized and the scale is indicated below the figures. ( a ) Unsupervised hierarchical clustering was performed for individual technical replicates. ( b ) Competitive inhibition of EV interactions with lectin microarray with mannose (Man) and N -acetylglucosamine (GlcNAc). ( c ) Lectin microarray of PNGase F-treated EVs. Significance (* = p ≤ 0.05) was determined by the ANOVA test.
Article Snippet: Probes (neoglyconjugates, glycoproteins and lectins) were printed on Nexterion® Slide H (Schott, Mainz, Germany) using a SciFlexArrayer S3 piezoelectric microarray printer (Scienion, Berlin, Germany) under constant 62% (+/−2%) humidity at 20 °C essentially as previously described .
Techniques: Inhibition, Microarray